![]() ![]() An, animal Veg, vegetal Dbl, dorsal blastopore lip Bp, blastopore. ( H–H’), ( I), ( M–N) Embryos are shown in the lateral view. ( M–M’) Enrichment of nuclear β-catenin and ( N) Axin mRNA in the dorsal endomesoderm of the embryos at early cap-shaped mid-gastrula stage. ( L) Axin is expressed at a higher level in the dorsal endomesoderm at early gastrula stage. ( K–K’) The signal of β-catenin, which is labeled with specific anti-amphioxus β-catenin antibody, is located in the dorsal endomesoderm. ![]() ( J’) β-catenin is located in the dorsal part of blastopore at early gastrula stage and co-expressed with Goosecoid protein ( J’’). Dashed lines demarcate presumptive dorsal endomesoderm. ( J) The dorsal part of blastopore of Branchiostoma laceoltum has a typical morphology and is different from the ventral part of blastopore at early gastrula stage. ( H–H’) Nuclear β-catenin and ( I) the expression of Axin are concentrated to the presumptive dorsal endomesoderm and dorsal blastopore lip at the onset of gastrulation. ( F–F’) Asymmetrical distribution of β-catenin and asymmetrical expression of Axin ( G) in the blastula of amphioxus embryo. ( E) The expression of the Wnt/β-catenin signaling target gene Axin is not ubiquitous in the 64-cell stage embryo. ( D–D’) Individual optical section demonstrates that β-catenin is present in the vegetal nuclei of a 64-cell stage embryo. ![]() Inserts in ( C) and ( C’) demonstrate individual optical sections from the vegetal and animal poles, respectively. ( B–B’) side view, ( C) vegetal view, ( C’) animal view. Arrowheads show polar body, which demarcates the animal pole. ( B–C’) Nuclear β-catenin is accumulated in the vegetal half of the embryo at 32-cell stage. ( A–A’) β-catenin is not present in the nuclei of the embryos at 8-cell stage. ( E), ( G), ( I), ( L) and ( N) In-situ hybridization of the Wnt/β-catenin signaling target gene Axin. ( K) Bright-field of the embryo shown with the signals in ( K’). ( J’’) Immunostaining of the same embryo as in ( J’) with specific anti-amphioxus Goosecoid antibody produced in the mouse. ( J’) Immunostaining with anti-human β-catenin antibody produced in the rabbit. ( A–D’), ( F–F’), ( H–H’), ( K’) and ( M–M’) Immunostaining of β-catenin with specific anti-amphioxus β-catenin antibody produced in the mouse. ( J) Bright-field of the embryo shown with the signals in ( J’) and ( J’’). ![]()
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